Neuroprotective Effect of Membrane-Free Stem Cell Extract against Amyloid Beta 25–35-Induced Neurotoxicity in SH-SY5Y Cells

Amyloid beta (A?) produced by the amyloidogenic pathway induces neurotoxicity, and its accumulation is a well-known cause of Alzheimer’s disease (AD). In this study, protective effect membrane-free stem cell extract (MFSCE) derived from adipose tissue against A?25–35-induced neurotoxicity in neuronal cells was investigated. Treatment with MFSCE increased viability decreased lactate dehydrogenase (LDH) release dose-dependent manner, compared group. The level reactive oxygen species (ROS) significantly induced A?25–35, whereas treatment dose-dependently reduced ROS production. attenuated neuroinflammation apoptosis downregulating inducible nitric oxide synthase, cyclooxygenase-2, B-cell lymphoma 2-associated X protein treated SH-SY5Y A?25–35. Furthermore, downregulated expression pathway-related proteins, such as amyloid precursor protein, ?-secretase, preselin-1, preselin-2. Therefore, study indicated neuroprotective suggesting that it useful strategy for AD.